Trigonella foenum-graecum L.

Last updated: 11 January 2017

Scientific Name

Trigonella foenum-graecum L.

Synonyms

Trigonella tibetana (Alef.) Vassilcz., Foenum-graecum officinale var. tibetanum Alef. [2]

Vernacular Name

Malaysia Halba [2]
English Fenugreek [2]

Geographical Distributions

Trigonella foenum-graecum is originated from India or the Middle East. It is now primarily grown in India and in the Mediterranean countries. This plant has been widely cultivated in Central and Southeastern Europe, Western Asia, India and Northern Africa. [3]

Botanical Description

T. foenum-graecum is a member of Leguminosae family. T. foenum-graecum is an erect, strongly aromatic, annual herb reaching 60 cm high. The leaves are trifoliate with a large petiole and leaflets 2-2.5 cm long. A stipule is found at the base of the petiole. The flowers are small and yellowish-white, borne singly or in pairs in the leaf axils. The fruit, a legume, which arises from the leaf axil, is 5-10 cm long, narrow, and pointed containing about 10-20 brownish-yellow seeds. [4]

Cultivation

No documentation

Chemical Constituent

T. foenum-graecum has been reported to contain protein (30%), lipids (5.5-7.5%), fatty acids which are dominantly linoleic acid (~40%) and linolenic acid. T. foenum-graecum alsohas been reported to contain galactomannans (15%), 4-Hydroxyisoleucine 1, a non-proteogenic amino-acid, (0.6%), pseudo alkaloid, trigonelline 2 (0.1-0.15%), phenolics: coumarins, flavonoids (~0.1%), over 50 volatile compounds, and flavour of the seeds is mainly provided by a small g-lactone, sotolone 3. [5] [6] Furthermore, T. foenum-graecum alsohas been reported to contain steroids and steroidal saponins: Free sterols, dominated by sitosterol, make about 0.2% of the seed weight. Steroidal saponins (4-6% of seed weight) include a homogenous series of steroidal sapogenins with furostan 4 and spirostan 5 skeletons and two glycosidic chains on C-3 and C-26, mucilage, tannicacid, fixed and vegetable oils, diosgenin, trigocoumarin, trigomethyl coumarin, steroidal saponin such as gitogenin and traces of trigogenin and vitamin A. [7][8][9]

Plant Part Used

Seeds. [2]

Traditional Use

T. foenum-graecum has been used in Indian and North African cuisine. As a traditional medicine, there is a variety of claims relative to its use. It is widely considered as antidiabetic and anticholesterol herb. It is used as a tonic and appetite stimulant in North Africa. In India, it is used to treat various GIT disorders. It is also said to be galactogogue and uterine stimulant. Its dried ripe seed is well known for its pungent aromatic properties and often used to add flavour to foods in Malaysian homes. [2][3]

Fenugreek also has been used in therapeutic treatment such as CNS stimulant, anti-ulcer, anti-inflammatory, intestinal pain, skin diseases, sexual impotence, wound healing, immunomodulatory, antioxidant, anti-neoplastic and anti-pyretic. [9][10]

Preclinical Data

Pharmacology

Hypoglycaemic activity

The hypoglycaemic activity of T. foenum-graecum was demonstrated on several animal models (normal mice and rats, insulin-dependant dogs, alloxan-induced diabetic mice, rats and dogs). [11][12][13] The activity was related to various components of the seeds. The soluble dietary fibre fraction, the main components of which are the galactomannans, was shown to reduce glycaemia after glucose administration [11]. This fraction was further shown to decrease the serum fructosamine level with no significant change in insulin level [14]. Another experiment using T. foenum-graecum mucilage administrated to streptozocin-induced diabetic rats confirmed the antidiabetic properties [15]. Trigonelline is known to have some hypoglycaemic effect. However, most attention was given to 4-hydroxyisoleucine. In vitro, it was shown to increase the insulin secretion by isolated Langerhans islets in a dose-dependant manner. It was also shown to induce a biphasic insulin response in isolated perfused rat pancreas. In vivo, it was shown to be effective in type 2 diabetic rats as well as in conscious fasted dogs in improving oral glucose tolerance after oral administration [11]. 4-Hydroxyisoleucine has been shown to increase glucose-induced insulin release without interacting with other agonists of insulin secretion such as tolbutamide and glyceraldehydes, thus demonstrating a novel in vitro insulinotropic activity [6]. The bio-molecular mechanism is still unclear. However, it was shown that seed powder was able to both increase the glutamate deshydrogenase and decrease that of D-b-hydroxybutyrate dehydrogenase in aloxanized rats. Ultrastructures of the rat liver showed a reduction in abnormalities [16]. An alcoholic seed extract exerted an anti-cataract in alloxanized diabetic rats [13]

Hypocholesterolemic activity

T. foenum-graecum showed some hypocholesterolemic activity. This activity was credited to both the galactomannans and the saponins. Galactomannans decrease the uptake of bile acids, lower blood and liver concentration of cholesterol and decrease hepatic cholesterol synthesis [11]. Indeed, the soluble dietary fibre fraction of the seeds significantly decreases the atherogenic lipids in type 2 diabetic rats [14]. Saponins were also shown to interact with bile salts in the GIT. [11][17]

Nutritive activity

Studies to verify these traditional claims provide somewhat contradictory results. Some studies using T. foenum-graecum food supplement did not alter the food intake of animals [18]. When given to type 2 diabetic patients, no significant changes were observed on the food consumption, mean energy intake or body weight. However, the seed extract was reported to increase appetite and food intake in rats. The saponins seem to be responsible for this effect, as a total saponin extract significantly increased appetite and body weight of normal as well as diabetic animals [11]

Gastroprotective activity

An aqueous extract and a gel fraction of T. foenum-graecum were shown to protect the gastric mucosa as efficiently and more efficiently, respectively than omeprazole in protecting rat from HCl-ethanol-induced gastric ulcers [19]

Antioxidant activity and chemopreventive activity

Consumption of an aqueous extract of T. foenum-graecum concurrently with ethanol for 60 days reduced liver and brain damage in rats compared with use of alcohol alone. The aqueous extract had an in vitro antioxidant potential in liver cells comparable to vitamin E and glutathione [20]. Ethanol-induced lipid peroxidation was prevented to rise in rats by an aqueous extract of fenugreek seeds. Similarly, during 1,2-dimethylhydrazine-induced rat colon carcinogenesis the circulatory lipid peroxidation was decreased and chemoprevention was exerted by adding T. foenum-graecum  to the diet [21]. A water extract of fenugreek seeds showed chemopreventive activity against 7,12-dimethylbenz-(a)-anthracene (DMBA)-induced breast cancer in rats [22]. A polyphenolic extract was able to prevent ethanol-induced cytotoxicity and apoptosis in Chang liver cells [23]. The same extract was also shown to protect erythrocytes from H2O2-induced oxidative damage. [7][24]

Antidiabetic activity

The studies reported the T. foenum-graecum has antidiabetic properties when its combine with sodium orthovanadate. The low dose in vanadate is mixed with fenugreek seed to prevent toxicity and antidiabetic effects are evaluated on membrane-linked functions and antioxidant enzymes in diabetic rat brains. Vanadium salts play a role as mimic of promoting effects of insulin. The previous study has reported diabetes reduced Na+/K+ ATPase activity in brain microsomal membrane. The 21 days after effectiveness treatment of Trigonella and 0.2 mg/ml vanadate was restored the reduced activity of Na+/K+ ATP, altered membrane fluidity and increased lipid peroxides. This alternative treatment can be explored further as a means of diabetic control. [8]

Immunomodulatory activity

T. foenum-graecum had showed some stimulatory effect on immune functions in mice [25].

Antiobesity activity

A laboratory animal study found that T. foenum-graecum is useful as antiobesity properties. The result reported that the body weight gain induced by high-fat diet in obese mice was decreased. It also has been elucidated the potential of fenugreek in inhibition of lipid accumulation in the liver without affected tissue weights of the kidney and spleen, whereas this process not involve toxicity activity of the extract. The absorption of triglyceride in the intestine also was investigated by a lipid-loading test to indicated reduction of body weight mechanism. The resulted was representing the corn oil administration caused reduction of plasma triglyceride. Also, 4-hydroxyisoleucine has the potential to decrease the plasma triglyceride gain by the same cause without reduce body weight gain induced by a high-fat diet. [26] In other study, the extract of sapogenins in the seeds of T. foenum-graecum is used to decrease the level of cholesterol as its potential to increase biliary secretion. [27][28]

Toxicity

T. foenum-graecum are considered as essentially non-toxic, either in acute or sub-chronic studies [18][29]. There is a report that links T. foenum-graecum (whole plant) with myopathy in ruminants [30].

Genotoxicity and Mutagenicity Studies

No genotoxicity found when T. foenum-graecum was evaluated by the standard battery of tests recommended by the US FDA for food ingredients. [31]

Clinical Data

Clinical findings

Hypoglycaemic activity

A study on cyclists showed that T. foenum-graecum extract increases insulin concentration and glycogen resynthesis after exercise [32]. Various clinical trials of T. foenum-graecum showed improvement in glucose tolerance in healthy volunteers as well as in type 2 and type 1 diabetic patients [33][34][35]. However, all these studies were considered as methodologically weak and classified as preliminary. [36][37]

Hypocholesterolemic activity

A clinical trial on hyperlipidemic non-diabetic patients whom diet was supplemented in defatted T. foenum-graecum showed significant reduction of serum total cholesterol, LDL and VLDL cholesterol and triglyceride levels, while HDL cholesterol levels were unchanged [11]. In another trial, T. foenum-graecum did not affect the blood profile in healthy subjects, but significantly reduced the total cholesterol and triglyceride without affecting the HDL-cholesterol in patients with coronary artery disease. [38] Similar results were obtained with germinated seeds, which are less bitter [39]. Long lasting hypo-cholesterolemic activity was also demonstrated in diabetic subjects. Altogether, the methodology of these studies was considered as poor [36][40]

Galactogogue activity

One study involving ten women indicated that T. foenum-graecum would increase milk production. However a stronger methodology would be required before this claim could be endorsed.

Side effects

High doses of T. foenum-graecum are associated with primarily gastrointestinal symptoms such as cramping, diarrhoea and flatulence. [41]

Pregnancy/Breast Feeding

It has been reported that T. foenum-graecum may increase lactation [42]. Ingestion of T. foenum-graecum by mothers during labour resulted in syrup-like odour in their new born infants, leading to a false suspicion of maple syrup urine disease (MSUD). This is due to the fact that T. foenum-graecum, maple syrup and urine of MSUD patients share sotolone as common component [43][44]. T. foenum-graecum is reputed to be oxytocic with in-vitro uterine stimulant activity. [45]

Adverse reaction

T. foenum-graecum has been reported to cause minor gastrointestinal symptoms (diarrhoea, flatulence) and allergy after inhalation of the seed powder or topical application of a seed powder paste. [46][47]

Interaction & Depletion

Interaction with drug

T. foenum-graecum has been reported to havepotential interaction with antiplatelet/anticoagulant drugs (incl. NSAIDs) where the presence of coumarin may theoretically increase the risk of haemorrhage (speculative). Interaction with exogenous insulin in diabetic patients who do not adjust insulin dosage which could lead to hypoglycaemia (speculative)and oral hypoglycaemic drugs sulfonylurea, biguanides, or both can lead to hypoglycaemia (speculative).T. foenum-graecum has been reported to havepotential enhancement of the activity of cholesterol-lowering agents due to additive effects (speculative). [48][49]

Interaction with other Herbs

T. foenum-graecum extract and garlic extract has been reported to be less effective in the regulation of hyperthyroidism in rats than administrated independently. [50]

Contraindications

No documentation

Case Report

Interaction with warfarin

There has been reported that a woman stabilized on warfarin developed an elevated INR after several weeks of using a capsule of T. foenum-graecum before meals and 10 drops after meals. Her INR returned to the normal range after stopping the herbal products but became elevated again after resuming their use. It may be that warfarin metabolism was reduced or the serum protein bond of warfarin was modified (speculative). [51]

Allergy

There has been reported that an Algerian student using T. foenum-graecum as an appetite stimulant and topically as healing agent developed a respiratory allergy. Handling this powder induced rhinitis and asthma. The prick test performed with fenugreek was strongly positive. [46]

There has been reported that a 36-year-old housewife experienced sneezing, rhinorrhea and excessive tearing after opening a jar containing T. foenum-graecum and smelling it for identification. This was followed by persistent coughing, wheezing and fainting. She was admitted to the hospital in a state of shock. The past history revealed allergy to chickpeas and occasional mild asthma. On the day the incident took place she had not consumed chickpeas. [47]

There has been reported that a 45-year-old woman with a history of allergic rhinitis and asthma since childhood developed pruritus and dandruff. On advice from a relative she applied T. foenum-graecum seed paste on her scalp. Within minutes, she developed nasal congestion and hoarseness. Later, she experienced facial angiodema, wheezing and numbness of the head. She was rushed to the hospital casualty ward. [47]

False maple syrup urine disease (MSUD)

There has been reported that a male infant was born at term by normal delivery weighing 3470 g, with Apgar score of 9 and 10 at 1 and 5 min, respectively. Several hours after birth, the nursing staff in the nursery noted a strong smell emanating from the skin and the diaper of the infant. The odour resembles maple syrup, leading the staff to suspect MSUD. Physical examination revealed an alert infant with normal vital signs, normal primitive reflexes and without any abnormal physical signs. All biological analyses yielded normal results. It was subsequently realized that the mother, of Yemenite Jewish origin, had ingested a spicy paste prepared from T. foenum-graecum seeds during the early hours of her labour. The same maple syrup-like odour could be detected on the mother’s skin. [43]

Dosage

No documentation

Poisonous Management

No documentation

Line drawing

No documentation

References

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