Antioxidant Activities of Mahkota Dewa (Phaleria Papuana) and Mangun (Coleus Amboinicus)
Medicinal and Aromatic Plants Seminar (MAPS 2008)
21st October - 22nd October 2008
The Legend Hotel, Kuala Lumpur
Methanol and water extracts of leaves and stems Coleus amboinicus (mahkota dewa) and leaves, fruits and seeds of Phaleria papuana (mangun) were used to investigate the antioxidant activities. Experiments were carried out using 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ferric thiocyanate (FTC) method and thiobarbituric acid (TBA) test to evaluate their antioxidant activities. The radical scavenging activity assay of the samples was determined from the reduction of the optical absorbance at 517 nm due to scavenging of the stable DPPH free radical. Results of the study showed that the strongest free radical scavenging activity were from the dried fruit of P.papuana (IC₅₀ = 98.2μg/ml) followed by its fresh fruit (IC₅₀ = 179.8μg/ml), leaves (IC₅₀ = 372.4μg/ml) and seeds (IC₅₀ = 431.5μg/ml), whereas, the leaves and stems of C. amboinicus showed weak free radical scavenging activity with IC₅₀ > 500 μg/ml. The amount of peroxide in the initial stages of lipid oxidant was measured in the FTC method. The methanol extracts showed the highest antioxidant activity in the order of P.papuana seeds > C.amboinicus stems > C. amboinicus leaves > P.papuana dried fruit > P.papuana leaves > P.papuana fresh fruits while the results for water extract of the samples showed a different pattern from the methanol extracts. It was in the order of P.papuana seeds > P.papuana fresh fruits > C.amboinicus stems > P.papuana dried fruit > P.papuana fruits > C. amboinicus leaves.
Coleus amboinicus (mahkota dewa); Phaleria papuana (mangun); plant extracts; antioxidant activiy assays
Think Global, Act Local: A New Paradigm In Malaysian Herbal Industry (Prof. Emeritus Dr. Abdul Latif Ibrahim-UNISEL)